Chemical germination of native and cation-exchanged bacterial spores with trifluoperazine.
نویسنده
چکیده
The calmodulin antagonist trifluoperazine and its analog chlorpromazine, both amphipaths, induced chemical germination of spores of various species, as do many surfactants. Cation load can greatly influence this response. Calmodulin antagonism does not seem to be involved. A new fluorometric assay for dipicolinic acid based on the fluorescence of the dipicolinic acid chelate of Tb3+ was found to be simple and sensitive.
منابع مشابه
Geobacillus stearothermophilus ATCC 7953 spore chemical germination mechanisms in model systems
Bacterial endospores through their strong resistance to both chemical and physical hurdles constitute a risk for food industry. Inactivation strategies are based on thermal and/or chemical treatments but rely on incomplete knowledge of the mechanisms of inactivation. Alternative strategies were suggested to achieve food safety while improving product quality. One of them relies on the successiv...
متن کاملThe duplication of bacterial chromatin; interpretations of some cytological and chemical studies of the germinating spores of Bacillus cereus and Bacillus megaterium.
In a separate paper, the changes in the P fractions of bacterial spores (luring geimination weere correlated with the morphological changes which took place at the same time. Germination has heen defined as the over-all period, some 50 to 60 min, extendling from the resting state of the "mononucleate" spore to the vegetative or "binucleate" cell. Thick suspensions (109 spores/ml) of synchronous...
متن کاملInduced release of dipicolinic acid from spores of Bacillus megaterium.
Conditions under which dipicolinic acid may be released from bacterial spores probably relate to the chemical state of the dipicolinic acid in the spore, to its physical location in the spore, and to its influence on the physiological properties of the spore. The latter aspect embraces the relation between release of dipicolinic acid and the viability of the spore, its heat resistance, and its ...
متن کاملGermination of bacterial spores by calcium chelates of dipicolinic acid analogues.
Calcium salts of analogues of dipicolinic acid were substituted for calcium dipicolinate in tests of germination of spores of Bacillus megaferium ATCC 10778. Only 4Hpyran-2,6-dicarboxylic acid was as active as dipicolinic acid. 3-Methyldipicolinic acid and 4-methyl-4H-pyran-2, B-dicarboxylic acid were active but the germination proceeded less rapidly. In the presence of a threshold concentratio...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 56 4 شماره
صفحات -
تاریخ انتشار 1990